4/29/2023 0 Comments Silica core shell nanoparticlesLangmuir isotherm age-dependent adsorption buffer interference with adsorption deoxynucleoside monophosphate nanocarrier nanoparticle solution NMR.Ī summary of the chemical synthesis steps ( a) and sorption optimization strategy ( b). We propose the prepared NPs as promising carriers for nucleic acid-type drug cargos. We studied the influence of biological buffers and found that while phosphate buffers decrease the loading dramatically, other commonly used buffers, such as HEPES, are compatible with the nanoplatform. The developed NPs bind as high as 207 mg dNMPs on 1 g of nanocarrier at neutral-physiological pH while maintaining good colloidal stability. The ligand adsorption was favored by acidic pH, while colloidal stability required neutral-alkaline pH thus, the optimal pH for the preparation of nucleic acid-modified particles is between 7.0-7.5. We determined the optimum conditions for adsorption of DNA building blocks, deoxynucleoside monophosphates (dNMP), the colloidal stability of the resulting NPs and its time dependence. We studied the behavior of the particles upon water dialysis and found that the ageing of the zirconia shell is a major determinant of the colloidal stability after transfer into the water due to physisorption of the zirconia starting material on the surface. We prepared high capacity 50 nm diameter NPs with monoclinic/cubic zirconia shell by a green, cheap and up-scalable sol-gel method. Nanoparticles (NP) in the size range of 20-200 nm have attractive properties as vaccine carriers because they achieve passive targeting of immune cells and can enhance the immune response of a weakly immunogenic antigen via their size. The development of delivery systems for the immobilization of nucleic acid cargo molecules is of prime importance due to the need for safe administration of DNA or RNA type of antigens and adjuvants in vaccines.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |